Journal: Scientific Reports

Article Title: The proteomic analysis of breast cell line exosomes reveals disease patterns and potential biomarkers

doi: 10.1038/s41598-020-70393-4

Figure Lengend Snippet: List of the membrane proteins suggested as potential breast cancer biomarkers.

Article Snippet: For the validation of protein biomarkers, isolated exosomes were incubated for 40 min with one of the following primary antibodies: Glut-1 (Invitrogen, MA5-31960, SA0377), GLYP-1 (Invitrogen, PA5-86043, polyclonal) and ADAM10 (MyBioSource, MBS435195, polyclonal).

Techniques: Membrane

Journal: Scientific Reports

Article Title: The proteomic analysis of breast cell line exosomes reveals disease patterns and potential biomarkers

doi: 10.1038/s41598-020-70393-4

Figure Lengend Snippet: Western blot analysis of CD63, CD81, GPC-1, GLUT-1, and ADAM10 in the exosomes derived from the MDA and MCF cell lines. Full-length gels are shown in Figure .

Article Snippet: For the validation of protein biomarkers, isolated exosomes were incubated for 40 min with one of the following primary antibodies: Glut-1 (Invitrogen, MA5-31960, SA0377), GLYP-1 (Invitrogen, PA5-86043, polyclonal) and ADAM10 (MyBioSource, MBS435195, polyclonal).

Techniques: Western Blot, Derivative Assay

Journal: Scientific Reports

Article Title: The proteomic analysis of breast cell line exosomes reveals disease patterns and potential biomarkers

doi: 10.1038/s41598-020-70393-4

Figure Lengend Snippet: tSNE plots of exosomes analyzed by FACS showing expression levels of ( a ) GPC-1 ( b ) GLUT-1 and ( c ) ADAM10 along with CD81 and CD63 on the surface of BC-derived exosomes.

Article Snippet: For the validation of protein biomarkers, isolated exosomes were incubated for 40 min with one of the following primary antibodies: Glut-1 (Invitrogen, MA5-31960, SA0377), GLYP-1 (Invitrogen, PA5-86043, polyclonal) and ADAM10 (MyBioSource, MBS435195, polyclonal).

Techniques: Expressing, Derivative Assay

Journal: eLife

Article Title: Proteomic landscape of tunneling nanotubes reveals CD9 and CD81 tetraspanins as key regulators

doi: 10.7554/eLife.99172

Figure Lengend Snippet:

Article Snippet: Antibody , Mouse monoclonal anti-ADAM10 11G2 , , Diaclone: #857.800.000 , WB (1/1000).

Techniques: Transfection, Construct, Expressing, Plasmid Preparation, Marker, Sequencing, Purification, Transduction, Control, Software, Staining

Journal: Oncoimmunology

Article Title: NKG2D- and T-cell receptor-dependent lysis of malignant glioma cell lines by human γδ T cells: Modulation by temozolomide and A disintegrin and metalloproteases 10 and 17 inhibitors

doi: 10.1080/2162402X.2015.1093276

Figure Lengend Snippet: Metalloprotease inhibition modulates ULBP2 cell surface expression and reduces ULBP2 shedding. Cells were treated for 24 h with the combination of the broad range MP inhibitors GM6001 (5 µM) and TAPI-1 (5 µM), or with GI (3 µM) or GW (3 µM) to inhibit ADAM10 and ADAM10/17, respectively. DMSO was used as a solvent control. (A) Cell surface expression of ULBP2 was determined by flow cytometry after treatment with MP inhibitors (filled histograms), with DMSO solvent control (open black histograms), or without treatment (medium control, open gray histograms). Dotted lines represent the isotype controls. (B) The concentration of sULBP2 in supernatants of medium control, DMSO- and inhibitor-treated GBM cells was determined by ELISA. The medium control was set to 100%. Data are presented as mean values of three to four independent experiments +/− SEM. Statistical significance is displayed as *** for p < 0.001; ** for p < 0.01 and * for p < 0.05.

Article Snippet: Anti-ADAM10 mAb (#857.800.000, clone 11G2) was obtained from Gen-Probe.

Techniques: Inhibition, Expressing, Flow Cytometry, Concentration Assay, Enzyme-linked Immunosorbent Assay